Working Group on CFTR Expression

European Working Group on CFTR Expression

The purpose of this website is to provide information which may be of interest to researchers in the fields of CFTR expression

and function. Currently this information includes:

Goals of the Working Group on CFTR Expression
Your Contribution is welcome
Annual Meetings
Applying for Training and Travel Grants
Scientific Committee and Coordinating Centre
Participants Apply for membership, it's free!
Acknowledgements

Goals of the CFTR Expression Workpackage

As part of the EU-sponsored Thematic Network around Cystic Fibrosis and Related Diseases (ended in January 2004), the

Workpackage devoted to resources for CFTR gene expression and function was funded. This Workpackage comprised the

following tasks:

To establish an European Network of researchers in the area of CFTR gene expression and function, grouped into several

expertise methodological areas. This Network will promote meetings so that researchers can share and discuss data from

ongoing and published CFTR studies in order to achieve consensus on those methodologies.

2. To set-up a system of reagent production and access, including a virtual repository (via a web site) of Biological Materials

to be used in CFTR expression studies.

3. To issue evidence-based consensus experimental protocols to improve result intercomparability, in the following

methodological expertise areas:

A. Transcript Analysis Qualitative and quantitative methods (RT-PCR, Northern, real-time PCR, etc.).

B. Cell Biology and Histology (immunocytochemistry, etc.).

C. Protein Biochemistry and Biophysics (Western blot, immunoprecipitation, pulse-chase, etc.).

D. Cell Physiology (SPQ assays, patch-clamp, etc.).

E. In vivo and ex vivo functional assessment (Ussing chamber analysis of biopsies, polyps, etc).

F. Animal and Cellular Models (cell lines, animal models, etc.).

To promote training and collaboration between groups to facilitate exchange of methodologies.
To disseminate and transfer relevant novel functional information (of diagnostic, prognostic, or therapeutic interest) from

the RTD setting to the users, i.e., health professionals in charge of patients with CF or related disorders. This information

transfer should result in a comprehensive improvement of current health care standards. In turn, it can also generate new

scientific questions.

Your Contribution is welcome

Contributions are welcome.

Please send an e-mail to cfnet@insa.min-saude.pt, (including your institutional address, e-mail, telephone and fax contact),

mentioning witch protocol (s) and/or resource (s) you would like to share.

Join the Working Group on CFTR Expression

If you want to become a member of the European Working Group on CFTR Expression, you will:

receive a Newsletter four times per year,
be informed about the annual meetings,
have acess to the mailing list, and
have the possibility to apply for our scientist exchange grants.

Membership ist free, please refer to: Becoming a member.

Upcoming Meetings

The European Cystic Fibrosis Society has decided to give continuity to these Lisbon meetings.

For more information visit: http://www.europeancfconference.org/lisbon.htm

Applying for Training and Travel Grants

These grants are no longer available

Coordinating Centre

Centro de Genética Humana
Instituto Nacional de Saúde Dr. Ricardo Jorge
Avenida Padre Cruz
P-1649-016 Lisboa
Portugal

Coordination

Margarida D. Amaral (Deputy)

Members of the Steering Committee

Acknowledgements

The Working Group on CFTR Expression is grateful for the excellent collaboration of Stephane Mazur, l'Association Française

de Lutte contre la Mucoviscidose (AFLM). He sent the "Registre des Outils et Matériels Biologiques Disponibles pour la Recherche

sur la Mucoviscidose", developed by the AFLM. We use it as an inspiration for the European CF Network catalogue and benefit

from the information collected by the AFLM.

URL: http://central.igc.gulbenkian.pt/cftr/index.html

Created: 2002-01-27